Subscribe to Updates

    Get the latest creative news from FooBar about art, design and business.

    What's Hot

    How To Choose The Right Lawyer For Your Claim Case?

    November 27, 2023

    Unlocking the Secrets of Successful Building Inspections for Homebuyers

    November 23, 2023

    GoTech Appliance Repairs and John H. Appliance Repair in Edmonton Keeping Your Tech in Top-Notch Shape!

    November 21, 2023
    Facebook Twitter Instagram
    Orkley
    • Home
    • Features
      • Example Post
      • Typography
      • Contact
      • View All On Demos
    • Site

      indian iphone storesinghtechcrunch

      February 16, 2023

      hoopla covid19 holocaustwoodcockvice

      February 16, 2023

      thestreamable

      February 16, 2023

      ali ghodsi

      February 16, 2023

      meam

      February 16, 2023
    • Typography
    • Mobiles
      1. Tech
      2. Gadgets
      3. Gaming
      4. View All

      Novel Solar Energy Harvesting System Breaks Efficiency Record

      October 22, 2022

      3D Printing Plant Cells Shows Promise for Studying Cell Function

      October 22, 2022

      Black Holes Can’t Trash Info About What They Swallow – and That’s a Problem

      October 22, 2022

      The Best Google Nest Mini Mounts and Stands in 2022

      October 22, 2022

      Tips And Tricks For Your DSLR Camera You Need To Know

      October 22, 2022

      By 2023, Digital Wallets are Expected to Surpass Cash Payment Mode

      October 22, 2022
      85

      Pico 4 Review: Should You Actually Buy One Instead Of Quest 2?

      January 20, 2021

      Get Massive Discounts on Xiaomi Redmi Note10 and AirDots

      January 15, 2021

      Call of Duty: Modern Warfare 2 PC Performance Analysis

      October 22, 2022

      Stranger Things, Dahmer Help Netflix Reach New Subscriber Record

      October 22, 2022

      This Alienware Gaming Laptop Just Got a Massive Price Cut

      October 22, 2022

      SkyTech Chronos Mini Gaming PC Drops Below $600

      October 22, 2022

      The New Rules for Registering IMEI Number for Tracking Lost Phone

      October 22, 2022

      Galaxy A54 is Tipped to Come With a Nominal Battery Upgrade in 2023

      October 22, 2022

      What Raw Materials are Used to Make Mobile Phones?

      October 22, 2022

      Production of iPhone 14 Costs 20% More Than iPhone 13

      October 22, 2022
    Orkley
    Home » Clinical Trial Essentials: The Difference Between Luminex Cytokine Assay And ELISA
    Health & Fitness

    Clinical Trial Essentials: The Difference Between Luminex Cytokine Assay And ELISA

    adminsideBy adminsideJune 8, 2022No Comments3 Mins Read
    Facebook Twitter Pinterest Telegram LinkedIn Tumblr WhatsApp Email
    Share
    Facebook Twitter LinkedIn Pinterest Telegram Email

    Cytokines are proteins or peptides produced by the immune system that are important in regulating the immune response. Cytokines regulate the maturation and responsiveness of the cell population, modulate immune responses, and are crucial mediators in health and disease states. Besides, the cytokine storm delivers insights into the pathophysiological processes and provides early indications of various diseases. Hence, determining cytokine behavior is crucial in understanding the complex signaling pathways in disease or normal states.

    Having said that, what is drug development? Drug development is the process of identifying potential drug compounds against disease and medical conditions. In recent years, Luminex assays have been widely used in early-stage drug development to assess cytokine behavior. Although Luminex assays are ideal for analyzing cytokines, ELISA has been traditionally used for evaluating target analytes in biological samples. However, Luminex assays have several advantages over ELISA. Let us understand the differences between ELISA and Luminex cytokine assay.

    Differences between Luminex cytokine assay and ELISA

    Compared to ELISA kits, Luminex assays are expensive and require specialized equipment. Therefore, understanding the difference between Luminex and ELISA assays is necessary to help researchers decide the best assay type for their individual research needs.

    The primary difference between ELISA and Luminex analysis is the use of different target capture and reporter systems. Luminex assays capture analytes on spherical beads, while ELISA uses microplates for capturing the target analytes. Fluorescence is the reporter system in Luminex assays, whereas ELISA employs enzymatic reactions of a colored substrate. Moreover, Luminex has digital signal processing capabilities to classify the dyed beads with different proportions of fluorophores. These distinct regions have a specific spectral signature in each bead population.

    Luminex analysis has several advantages over traditional ELISA and microarrays. Each microsphere of a Luminex assay is conceptually an ELISA on a bead. This system means that each bead is equal to an individual ELISA assay. Such functionality enables the analysis of 100 different targets in a single well of Luminex assays. Besides, the 3D structure and smaller surface area empower faster reaction kinetics and reduce the run time compared to ELISA assays.

    Besides, custom Luminex assays allow the attachment of specific probes that can rapidly expand and adapt to mutations in the population, making it an ideal screening tool. Furthermore, Luminex requires only 50 μl of sample assay volume per well. This assay volume is relatively low compared to ELISA assays. Hence, more data can be obtained from limited sample volumes. Moreover, analysis of samples such as cerebrospinal fluid that are difficult to obtain or are present at low volumes becomes easy with Luminex assays.

    Compared to ELISA assays, Luminex analysis provides both high-throughput and high-density analysis with reliable, reproducible, and accurate results. Although ELISA can provide high-throughput analysis, it can’t multiplex more than a few tests at a given time. On the other hand, microarrays can provide high-density screening but lack the reproducibility required for high-throughput analysis.

    However, there are several factors to consider before using Luminex cytokine assays. Luminex assays require dedicated instruments, which may increase the upfront costs of Luminex analysis. Although, the development of light-emitting diode-based systems can help decrease the setup costs. Besides, differences in assay manufacturer, assay execution, and product lot number can cause variability in Luminex results. Additionally, human autoantibodies in biological samples may hinder the measurement of cytokines in biological samples.

    luminex cytokine assay
    Share. Facebook Twitter Pinterest LinkedIn Tumblr Email
    adminside
    • Website

    Related Posts

    What You Need to Know Before Buying Mobility Equipment

    November 9, 2023

    Comprehensive Guide: Is CBD Vape Good for Sleep?

    October 24, 2023

    Unveiling the Benefits and Uses of CBD BUD: Your Ultimate Resource

    October 9, 2023

    CBD Chocolate: What to Look for and Why You Should Incorporate It into Your Routine

    May 16, 2023

    Is Marijuana Use Sustainable and Safe?

    January 26, 2023

    Are CBD Gummies Good For Tinnitus?

    January 20, 2023

    Comments are closed.

    Top Reviews
    9.1
    Editor's Choice

    Review: Mi 10 Mobile with Qualcomm Snapdragon 870 Mobile Platform

    By adminside
    8.9
    Uncategorized

    Smart Home Décor : Technology Offers a Slew of Options

    By adminside
    8.9
    Editor's Choice

    Edifier W240TN Earbud Review: Fancy Specs Aren’t Everything

    By adminside
    Editors Picks

    How To Choose The Right Lawyer For Your Claim Case?

    November 27, 2023

    Unlocking the Secrets of Successful Building Inspections for Homebuyers

    November 23, 2023

    GoTech Appliance Repairs and John H. Appliance Repair in Edmonton Keeping Your Tech in Top-Notch Shape!

    November 21, 2023

    4 Impressive Tips for Moving into Your New Warehouse

    November 16, 2023
    Advertisement
    Demo
    About Us
    Our Picks

    How To Choose The Right Lawyer For Your Claim Case?

    November 27, 2023

    Unlocking the Secrets of Successful Building Inspections for Homebuyers

    November 23, 2023

    GoTech Appliance Repairs and John H. Appliance Repair in Edmonton Keeping Your Tech in Top-Notch Shape!

    November 21, 2023
    Top Reviews
    9.1

    Review: Mi 10 Mobile with Qualcomm Snapdragon 870 Mobile Platform

    January 15, 2021
    8.9

    Smart Home Décor : Technology Offers a Slew of Options

    January 15, 2021
    8.9

    Edifier W240TN Earbud Review: Fancy Specs Aren’t Everything

    January 15, 2021
    All rights are reserved
    • Home
    • Tech
    • Gadgets
    • Mobiles

    Type above and press Enter to search. Press Esc to cancel.